|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Nuclear Transcription Factor Y, beta (NFYB) antibody
|Synonyms||HAP3, CBF-A, CBF-B, NF-YB, NFYB, zgc:110533, zgc:110552, hap3, cbf-a, cbf-b|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||NFYB (AAH05317, 1 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: CBFA, CBFB, HAP3, NFYB nuclear transcription factor Y, beta|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||NFYB (AAH05317, 1 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||22831 DA|
Background: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Transcription Factors, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged NFYB is approximately 0.03ng/ml as a capture antibody.|
Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).
Di Agostino, Strano, Emiliozzi et al.: "Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation." in: Cancer cell, Vol. 10, Issue 3, pp. 191-202, 2006 (PubMed).
Kang, Kim, Chang et al.: "CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A." in: Journal of cellular biochemistry, Vol. 104, Issue 4, pp. 1192-203, 2008 (PubMed).
Raju, Göritz, Nord et al.: "In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 3008-19, 2008 (PubMed).
Stros, Polanská, Struncová et al.: "HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha." in: Nucleic acids research, Vol. 37, Issue 7, pp. 2070-86, 2009 (PubMed).
|Hosts||Rabbit (16), Mouse (4)|
|Reactivities||Human (20), Mouse (Murine) (4), Rat (Rattus) (2), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (17), ELISA (13), Immunoprecipitation (IP) (8), Electrophoretic Mobility-Shift Assay (EMSA) (2), Gel Shift (GS) (2), Immunocytochemistry (ICC) (2), Immunofluorescence (IF) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Dot Blot (Dot) (1)|
|Epitopes||N-Term (9), Internal Region (1)|