Nuclear Transcription Factor Y, beta (NFYB) antibody

Antigen: Nuclear Transcription Factor Y, beta (NFYB)

Synonyms: HAP3, CBF-A, CBF-B, NF-YB, NFYB, zgc:110533, zgc:110552, hap3, cbf-a, cbf-b

Clonality: Monoclonal (5D1)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394392

Additional Information

Alternative name: NFYB

Gene ID: 4801

Immunogen: NFYB (AAH05317, 1 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5D1

Description: Other names: CBFA, CBFB, HAP3, NFYB nuclear transcription factor Y, beta

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: NFYB (AAH05317, 1 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 22831 DA

Comments:

Background: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged NFYB is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Di Agostino, Strano, Emiliozzi et al.: "Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation." in: Cancer cell, Vol. 10, Issue 3, pp. 191-202, 2006 (PubMed).

Kang, Kim, Chang et al.: "CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A." in: Journal of cellular biochemistry, Vol. 104, Issue 4, pp. 1192-203, 2008 (PubMed).

Raju, Göritz, Nord et al.: "In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 3008-19, 2008 (PubMed).

Stros, Polanská, Struncová et al.: "HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha." in: Nucleic acids research, Vol. 37, Issue 7, pp. 2070-86, 2009 (PubMed).