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Centromere Protein J (CENPJ) antibody

Details for Product No. ABIN394394
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Antigen
Synonyms BM032, CENP-J, CPAP, LAP, LIP1, MCPH6, SASS4, SCKL4, Sas-4, 4932437H03Rik, Gm81, CENPJ, fi46g11, wu:fi46g11, si:dkey-46k9.9
Reactivity
Human
(24), (2), (2)
Host
Mouse
(13), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(21), (16), (7), (4), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394394
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen CENPJ (NP_060921, 979 a.a. ~ 1089 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5D5
Isotype IgG2a kappa
Specificity CENPJ (NP_060921, 979 a.a. ~ 1089 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CENPJ
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 153000 DA
Gene ID 12406
UniProt NP_060921
Research Area Cell Signaling, Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Centromere Protein J (CENPJ) antibody Immunoperoxidase of monoclonal antibody to CENPJ on formalin-fixed paraffin-embedded human pancreas (antibody concentration 1.5 µg/mL). Detection limit for recombinant GST tagged CENPJ is approximately 0.03 ng/mL as a capture antibody.
anti-Centromere Protein J (CENPJ) antibody (2) anti-Centromere Protein J (CENPJ) antibody (Image 2)
Background publications Tang, Fu, Wu et al.: "CPAP is a cell-cycle regulated protein that controls centriole length." in: Nature cell biology, Vol. 11, Issue 7, pp. 825-31, 2009 (PubMed).

Zhao, Jin, Chu et al.: "Dimerization of CPAP orchestrates centrosome cohesion plasticity." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2488-97, 2010 (PubMed).

Rimol, Agartz, Djurovic et al.: "Sex-dependent association of common variants of microcephaly genes with brain structure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 1, pp. 384-8, 2010 (PubMed).

Al-Dosari, Shaheen, Colak et al.: "Novel CENPJ mutation causes Seckel syndrome." in: Journal of medical genetics, Vol. 47, Issue 6, pp. 411-4, 2010 (PubMed).

Chang, Cizmecioglu, Hoffmann et al.: "PLK2 phosphorylation is critical for CPAP function in procentriole formation during the centrosome cycle." in: The EMBO journal, Vol. 29, Issue 14, pp. 2395-406, 2010 (PubMed).

Hosts (13), (11)
Reactivities (24), (2), (2)
Applications (21), (16), (7), (4), (3), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1)
Epitopes (6), (5), (3), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN394394
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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