Centromere Protein J (CENPJ) antibody

Details for Product No. ABIN394394
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Antigen
Synonyms BM032, CENP-J, CPAP, LAP, LIP1, MCPH6, SASS4, SCKL4, Sas-4, 4932437H03Rik, Gm81, CENPJ, fi46g11, wu:fi46g11, si:dkey-46k9.9
Reactivity
Human
(6), (2), (2)
Host
Mouse
(5), (1)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(4), (4), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN394394
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen CENPJ (NP_060921, 979 a.a. ~ 1089 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5D5
Isotype IgG2a kappa
Specificity CENPJ (NP_060921, 979 a.a. ~ 1089 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CENPJ
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 153000 DA
Gene ID 12406
UniProt NP_060921
Research Area Cell Signaling, Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Centromere Protein J (CENPJ) antibody Immunoperoxidase of monoclonal antibody to CENPJ on formalin-fixed paraffin-embedded human pancreas (antibody concentration 1.5 µg/mL). Detection limit for recombinant GST tagged CENPJ is approximately 0.03 ng/mL as a capture antibody.
anti-Centromere Protein J (CENPJ) antibody (2) anti-Centromere Protein J (CENPJ) antibody (Image 2)
Background publications Tang, Fu, Wu et al.: "CPAP is a cell-cycle regulated protein that controls centriole length." in: Nature cell biology, Vol. 11, Issue 7, pp. 825-31, 2009 (PubMed).

Zhao, Jin, Chu et al.: "Dimerization of CPAP orchestrates centrosome cohesion plasticity." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2488-97, 2010 (PubMed).

Rimol, Agartz, Djurovic et al.: "Sex-dependent association of common variants of microcephaly genes with brain structure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 1, pp. 384-8, 2010 (PubMed).

Al-Dosari, Shaheen, Colak et al.: "Novel CENPJ mutation causes Seckel syndrome." in: Journal of medical genetics, Vol. 47, Issue 6, pp. 411-4, 2010 (PubMed).

Chang, Cizmecioglu, Hoffmann et al.: "PLK2 phosphorylation is critical for CPAP function in procentriole formation during the centrosome cycle." in: The EMBO journal, Vol. 29, Issue 14, pp. 2395-406, 2010 (PubMed).

Hosts (5), (1)
Reactivities (6), (2), (2)
Applications (4), (4), (2), (1), (1), (1)
Epitopes (2)
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