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STON1 antibody (Stonin 1) (AA 141-250)

Details for Product anti-STON1 Antibody No. ABIN394397, Supplier: Log in to see
Antigen
  • 4921524J06Rik
  • C86231
  • DKFZP469K176
  • Salf
  • salf
  • SALF
  • SBLF
  • Sblf
  • STN1
  • STNB1
  • Xsal2
  • XsalF
  • XsalF-a
  • XsalF-b
Epitope
AA 141-250
5
3
2
2
2
1
1
1
1
Reactivity
Human
21
2
2
1
1
1
1
Host
Mouse
16
5
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
19
12
4
2
2
Supplier
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Immunogen SALF (NP_758515, 141 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5F12
Isotype IgG2a kappa
Specificity SALF (NP_758515, 141 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SALF (STON1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 83141 DA
Gene ID 12406
NCBI Accession NP_758515
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The STON1-GTF2A1L mRNA is an infrequent but naturally occurring readthrough product of the neighboring STON1 and GTF2A1L genes. This rare transcript encodes a fusion protein composed of greater than 95% each of the individual elements, stonin 1 and general transcription factor IIA, 1-like. The significance of this readthrough mRNA and the function of its protein product have not yet been determined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-STON1 antibody (Stonin 1) (AA 141-250) (ABIN394397) Detection limit for recombinant GST tagged SALF is approximately 0.03 ng/mL as a capt...
Background publications Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Walther, Krauss, Diril, Lemke, Ricotta, Honing, Kaiser, Haucke: "Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating." in: EMBO reports, Vol. 2, Issue 7, pp. 634-40, 2001 (PubMed).

Martina, Bonangelino, Aguilar, Bonifacino: "Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery." in: The Journal of cell biology, Vol. 153, Issue 5, pp. 1111-20, 2001 (PubMed).

Han, Zhou, Upadhyaya, Lee, Parker, DeJong: "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse." in: Biology of reproduction, Vol. 64, Issue 2, pp. 507-17, 2001 (PubMed).

Upadhyaya, Lee, DeJong: "Identification of a general transcription factor TFIIAalpha/beta homolog selectively expressed in testis." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 18040-8, 1999 (PubMed).