Stonin 1 (STON1) (AA 141-250) antibody

Details for Product No. ABIN394397
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Antigen
Synonyms DKFZP469K176, Xsal2, XsalF, XsalF-a, XsalF-b, salf, SALF, 4921524J06Rik, C86231, Salf, Sblf, SBLF, STN1, STNB1
Epitope
AA 141-250
(5), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(21), (1), (1), (1), (1), (1), (1)
Host
Mouse
(16), (5)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
(20), (13), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394397
450.00 $
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Immunogen SALF (NP_758515, 141 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5F12
Isotype IgG2a kappa
Specificity SALF (NP_758515, 141 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SALF
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 83141 DA
Gene ID 12406
NCBI Accession NP_758515
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The STON1-GTF2A1L mRNA is an infrequent but naturally occurring readthrough product of the neighboring STON1 and GTF2A1L genes. This rare transcript encodes a fusion protein composed of greater than 95% each of the individual elements, stonin 1 and general transcription factor IIA, 1-like. The significance of this readthrough mRNA and the function of its protein product have not yet been determined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Stonin 1 (STON1) (AA 141-250) antibody Detection limit for recombinant GST tagged SALF is approximately 0.03 ng/mL as a capture antibody.
Background publications Upadhyaya, Lee, DeJong: "Identification of a general transcription factor TFIIAalpha/beta homolog selectively expressed in testis." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 18040-8, 1999 (PubMed).

Han, Zhou, Upadhyaya et al.: "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse." in: Biology of reproduction, Vol. 64, Issue 2, pp. 507-17, 2001 (PubMed).

Martina, Bonangelino, Aguilar et al.: "Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery." in: The Journal of cell biology, Vol. 153, Issue 5, pp. 1111-20, 2001 (PubMed).

Walther, Krauss, Diril et al.: "Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating." in: EMBO reports, Vol. 2, Issue 7, pp. 634-40, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

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