T antibody

Antigen: T

Clonality: Monoclonal (5H8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394406

Additional Information

Gene ID: 6862

UniProt: NP_003172

Immunogen: T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5H8

Description: Other names: MGC104817, TFT T, brachyury homolog (mouse)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 47443 DA

Comments:

Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of T over-expressed 293 cell line, cotransfected with T Validated Chimera RNAi ( Cat # H00006862-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with T monoclonal antibody (M01), clone 5H8 (Cat # AT4130a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M01), clone 5H8. Lane 1: T transfected lysate(41.1 KDa). Lane 2: Non-transfected lysate.

anti-T antibody (Image 2)

Publications

Product: Bondue, Lapouge, Paulissen et al.: "Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification." in: Cell stem cell, Vol. 3, Issue 1, pp. 69-84, 2008 (PubMed).

Oakley, Fuhrer, Seethala: "Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential: a tissue microarray-based comparative analysis." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 12, pp. 1461-9, 2008 (PubMed).

Yang, Ng, Alcorta et al.: "T (brachyury) gene duplication confers major susceptibility to familial chordoma." in: Nature genetics, Vol. 41, Issue 11, pp. 1176-8, 2009 (PubMed).

Docherty, Kovas, Petrill et al.: "Generalist genes analysis of DNA markers associated with mathematical ability and disability reveals shared influence across ages and abilities." in: BMC genetics, Vol. 11, pp. 61, 2010 (PubMed).

Jambhekar, Rekhi, Thorat et al.: "Revisiting chordoma with brachyury, a "new age" marker: analysis of a validation study on 51 cases." in: Archives of pathology & laboratory medicine, Vol. 134, Issue 8, pp. 1181-7, 2010 (PubMed).