T antibody

Details for Product No. ABIN394406
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Antigen
Reactivity
Human
(10), (1)
Host
Mouse
(6), (4)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(7), (6), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394406
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5H8
Isotype IgG2a kappa
Specificity T (NP_003172, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 47443 DA
Gene ID 12406
UniProt NP_003172
Research Area Transcription Factors, Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-T antibody Western blot analysis of T over-expressed 293 cell line, cotransfected with T Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M01), clone 5H8. Lane 1: T transfected lysate(41.1 KDa). Lane 2: Non-transfected lysate.
anti-T antibody (2) anti-T antibody (Image 2)
Background publications Bondue, Lapouge, Paulissen et al.: "Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification." in: Cell stem cell, Vol. 3, Issue 1, pp. 69-84, 2008 (PubMed).

Oakley, Fuhrer, Seethala: "Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential: a tissue microarray-based comparative analysis." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 12, pp. 1461-9, 2008 (PubMed).

Yang, Ng, Alcorta et al.: "T (brachyury) gene duplication confers major susceptibility to familial chordoma." in: Nature genetics, Vol. 41, Issue 11, pp. 1176-8, 2009 (PubMed).

Docherty, Kovas, Petrill et al.: "Generalist genes analysis of DNA markers associated with mathematical ability and disability reveals shared influence across ages and abilities." in: BMC genetics, Vol. 11, pp. 61, 2010 (PubMed).

Jambhekar, Rekhi, Thorat et al.: "Revisiting chordoma with brachyury, a \new age\" marker: analysis of a validation study on 51 cases."" in: Archives of pathology & laboratory medicine, Vol. 134, Issue 8, pp. 1181-7, 2010 (PubMed).

Hosts (6), (4)
Reactivities (10), (1)
Applications (7), (6), (2), (2), (1)
Epitopes (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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