Upstream Transcription Factor 2, C-Fos Interacting (USF2) antibody

Antigen: Upstream Transcription Factor 2, C-Fos Interacting (USF2)

Synonyms: FIP, bHLHb12, fip, MGC79773, bhlhb12, USF2, fb12g11, wu:fb12g11

Clonality: Monoclonal (6A9)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394409

Additional Information

Alternative name: USF2

Gene ID: 7392

UniProt: NP_003358

Immunogen: USF2 (NP_003358, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 6A9

Description: Other names: FIP upstream transcription factor 2, c-fos interacting

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: USF2 (NP_003358, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 36955 DA

Comments:

Background: This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to USF2 (AT4480a) on HeLa cell . [antibody concentration 10 ug/ml] USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in different cell lines.

anti-Upstream Transcription Factor 2, C-Fos Interacting (USF2) antibody (Image 2)

Publications

Product: Agirre, Jiménez-Velasco, San José-Enériz et al.: "Down-regulation of hsa-miR-10a in chronic myeloid leukemia CD34+ cells increases USF2-mediated cell growth." in: Molecular cancer research : MCR, Vol. 6, Issue 12, pp. 1830-40, 2008 (PubMed).

Finotti, Treves, Zorzato et al.: "Upstream stimulatory factors are involved in the P1 promoter directed transcription of the A beta H-J-J locus." in: BMC molecular biology, Vol. 9, pp. 110, 2009 (PubMed).

Wang, Li, Zhang et al.: "HINT1 inhibits beta-catenin/TCF4, USF2 and NFkappaB activity in human hepatoma cells." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 7, pp. 1526-34, 2009 (PubMed).

Landa, Ruiz-Llorente, Montero-Conde et al.: "The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors." in: PLoS genetics, Vol. 5, Issue 9, pp. e1000637, 2009 (PubMed).

Sun, Yu, Degraff et al.: "Upstream stimulatory factor 2, a novel FoxA1-interacting protein, is involved in prostate-specific gene expression." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 12, pp. 2038-47, 2009 (PubMed).