Activating Transcription Factor 3 (ATF3) antibody

Antigen: Activating Transcription Factor 3 (ATF3)

Synonyms: LRFI, LRF-1, LRG-21, MGC55526, zgc:55526, atf-3, MGC81814, ATF3, MGC137608

Clonality: Monoclonal (6B8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394412

Additional Information

Alternative name: ATF3

Gene ID: 467

Immunogen: ATF3 (AAH06322, 1 a.a. ~ 182 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa

Clone: 6B8

Description: Other names: - activating transcription factor 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ATF3 (AAH06322, 1 a.a. ~ 182 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 20576 DA

Comments:

Background: Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ATF3 over-expressed 293 cell line, cotransfected with ATF3 Validated Chimera RNAi ( Cat # H00000467-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF3 monoclonal antibody (M01), clone 6B8 (Cat # AT1222a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of ATF3 expression in transfected 293T cell line by ATF3 monoclonal antibody (M01), clone 6B8. Lane 1: ATF3 transfected lysate(20.6 KDa). Lane 2: Non-transfected lysate.

anti-Activating Transcription Factor 3 (ATF3) antibody (Image 2)

Publications

Product: Filén, Ylikoski, Tripathi et al.: "Activating transcription factor 3 is a positive regulator of human IFNG gene expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4990-9, 2010 (PubMed).

Nguyen, Johnsen, Knetter et al.: "Differential gene expression downstream of Toll-like receptors (TLRs): role of c-Src and activating transcription factor 3 (ATF3)." in: The Journal of biological chemistry, Vol. 285, Issue 22, pp. 17011-9, 2010 (PubMed).

Koh, Lim, Joe et al.: "AdipoR2 is transcriptionally regulated by ER stress-inducible ATF3 in HepG2 human hepatocyte cells." in: The FEBS journal, Vol. 277, Issue 10, pp. 2304-17, 2010 (PubMed).

Wu, Nguyen, Dziunycz et al.: "Opposing roles for calcineurin and ATF3 in squamous skin cancer." in: Nature, Vol. 465, Issue 7296, pp. 368-72, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).