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Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) (AA 9-119) antibody

Details for Product No. ABIN394425, Supplier: Log in to see
Antigen
  • 0610040H15Rik
  • cb162
  • wu:fb47e08
  • ELOB
  • SIII
Epitope
AA 9-119
16
16
15
11
8
3
2
2
2
1
1
1
1
1
Reactivity
Human
57
26
10
2
1
1
Host
Mouse
55
17
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
4
4
4
4
4
1
Application
Immunofluorescence (IF), Western Blotting (WB)
61
42
13
8
2
2
2
1
1
1
1
1
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Immunogen TCEB2 (NP_009039, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6F6
Isotype IgG2a kappa
Specificity TCEB2 (NP_009039, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TCEB2 (TCEB2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 13133 DA
Gene ID 12406
NCBI Accession NP_009039
Research Area Cancer, Transcription Factors, Signaling, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) (AA 9-119) antibody (ABIN394425) Immunofluorescence of monoclonal antibody to TCEB2 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) (AA 9-119) antibody (ABIN394425) anti-Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) (AA 9-119) antibody (Image 2)
Background publications Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Hacker, Lee, Rathmell: "VHL type 2B mutations retain VBC complex form and function." in: PLoS ONE, Vol. 3, Issue 11, pp. e3801, 2008 (PubMed).

Piessevaux, De Ceuninck, Catteeuw et al.: "Elongin B/C recruitment regulates substrate binding by CIS." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21334-46, 2008 (PubMed).

Menon, Tsuge, Dohmae et al.: "Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome." in: BMC biochemistry, Vol. 9, pp. 1, 2008 (PubMed).

Ferguson, Wu, Smith et al.: "ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism." in: Molecular and cellular biology, Vol. 27, Issue 18, pp. 6407-19, 2007 (PubMed).