Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) antibody

Antigen: Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2)

Synonyms: ELOB, SIII

Clonality: Monoclonal (6F6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394425

Additional Information

Alternative name: TCEB2

Gene ID: 6923

UniProt: NP_009039

Immunogen: TCEB2 (NP_009039, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 6F6

Description: Other names: SIIItranscription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TCEB2 (NP_009039, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 13133 DA

Comments:

Background: This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to TCEB2 (AT4179a) on HeLa cell . [antibody concentration 10 ug/ml] TCEB2 monoclonal antibody (M01), clone 6F6 Western Blot analysis of TCEB2 expression in Hela NE ( Cat # L013V3 ).

anti-Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) antibody (Image 2)

Publications

Product: Ferguson, Wu, Smith et al.: "ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism." in: Molecular and cellular biology, Vol. 27, Issue 18, pp. 6407-19, 2007 (PubMed).

Menon, Tsuge, Dohmae et al.: "Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome." in: BMC biochemistry, Vol. 9, pp. 1, 2008 (PubMed).

Piessevaux, De Ceuninck, Catteeuw et al.: "Elongin B/C recruitment regulates substrate binding by CIS." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21334-46, 2008 (PubMed).

Hacker, Lee, Rathmell: "VHL type 2B mutations retain VBC complex form and function." in: PLoS ONE, Vol. 3, Issue 11, pp. e3801, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).