Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN394425

Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ELOB, SIII, 0610040H15Rik, cb162, wu:fb47e08
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN394425
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen TCEB2 (NP_009039, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6F6
Isotype IgG2a kappa
Specificity TCEB2 (NP_009039, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TCEB2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 13133 DA
Gene ID 12406
UniProt NP_009039
Research Area Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence
Comment

Background: This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Ferguson, Wu, Smith et al.: "ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism." in: Molecular and cellular biology, Vol. 27, Issue 18, pp. 6407-19, 2007 (PubMed).

Menon, Tsuge, Dohmae et al.: "Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome." in: BMC biochemistry, Vol. 9, pp. 1, 2008 (PubMed).

Piessevaux, De Ceuninck, Catteeuw et al.: "Elongin B/C recruitment regulates substrate binding by CIS." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21334-46, 2008 (PubMed).

Hacker, Lee, Rathmell: "VHL type 2B mutations retain VBC complex form and function." in: PLoS ONE, Vol. 3, Issue 11, pp. e3801, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Alternatives for antigen "Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2)", type "Antibodies"
Hosts (26), (11), (3)
Reactivities (37), (19), (17), (13), (12), (12), (1)
Applications (29), (14), (10), (6), (3), (3), (2), (1), (1)
Conjugates (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (12), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Beacon