Nuclear Transcription Factor Y, beta (NFYB) antibody

Details for Product No. ABIN394430
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Antigen
Synonyms HAP3, CBF-A, CBF-B, NF-YB, nfyb, zgc:110533, AA985999, Cbf-A, CBFB, Nfya, NFYB, hap3, cbf-a, cbf-b
Reactivity
Human
(40), (9), (7), (2), (2), (2), (1), (1), (1)
Host
Mouse
(28), (12)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(35), (24), (11), (10), (4), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN394430
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen NFYB (AAH05317, 1 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6H6
Isotype IgG2a kappa
Specificity NFYB (AAH05317, 1 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NFYB
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 22831 DA
Gene ID 12406
Research Area Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Nuclear Transcription Factor Y, beta (NFYB) antibody Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6. Lane 1: NFYB transfected lysate(22.8 KDa). Lane 2: Non-transfected lysate. Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi used as specificity and loading control.
anti-Nuclear Transcription Factor Y, beta (NFYB) antibody (2) anti-Nuclear Transcription Factor Y, beta (NFYB) antibody (Image 2)
Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Di Agostino, Strano, Emiliozzi et al.: "Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation." in: Cancer cell, Vol. 10, Issue 3, pp. 191-202, 2006 (PubMed).

Kang, Kim, Chang et al.: "CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A." in: Journal of cellular biochemistry, Vol. 104, Issue 4, pp. 1192-203, 2008 (PubMed).

Raju, Göritz, Nord et al.: "In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 3008-19, 2008 (PubMed).

Stros, Polanská, Struncová et al.: "HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha." in: Nucleic acids research, Vol. 37, Issue 7, pp. 2070-86, 2009 (PubMed).

Hosts (28), (12)
Reactivities (40), (9), (7), (2), (2), (2), (1), (1), (1)
Applications (35), (24), (11), (10), (4), (4), (2), (2), (2), (2), (1), (1), (1)
Epitopes (14), (6), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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