3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1) antibody

Antigen: 3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1)

Synonyms: PIP, BPntase, Sal3, MGC93112, MGC82412, BPNT1, MGC126947

Clonality: Monoclonal (2E1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394459

Additional Information

Alternative name: BPNT1

Gene ID: 10380

UniProt: NP_006076

Immunogen: BPNT1 (NP_006076, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2E1

Description: Other names: PIP 3'(2'), 5'-bisphosphate nucleotidase 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BPNT1 (NP_006076, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 33392 DA

Comments:

Background: BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to BPNT1 (AT1310a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of BPNT1 expression in transfected 293T cell line by BPNT1 monoclonal antibody (M01), clone 2E1. Lane 1: BPNT1 transfected lysate(28.1 KDa). Lane 2: Non-transfected lysate.

anti-3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1) antibody (Image 2)

Publications

Product: Spiegelberg, Xiong, Smith et al.: "Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate." in: The Journal of biological chemistry, Vol. 274, Issue 19, pp. 13619-28, 1999 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).