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3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1) antibody

Antigen

3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1)

Synonyms PIP, BPntase, Sal3, MGC93112, MGC82412, BPNT1, MGC126947
Clonality Monoclonal (2E1)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
5 references available
Certificates ISO 9001:2008
Catalog no. ABIN394459
Quantity 0.1mg
Price 450.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name BPNT1
Gene ID 10380
UniProt NP_006076
Immunogen BPNT1 (NP_006076, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Isotype IgG1 kappa
Clone 2E1
Description Other names: PIP 3'(2'), 5'-bisphosphate nucleotidase 1
Characteristics Purified Mouse Monoclonal Antibody (Mab)
Specificity BPNT1 (NP_006076, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Molecular Weight 33392 DA
Comments

Background: BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity.

Application Details

Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence
Purification Purified
Buffer Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Cell Signaling, Metabolism, Cell Structure
Restrictions For Research Use only

Publications

Product Spiegelberg, Xiong, Smith et al.: "Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate." in: The Journal of biological chemistry, Vol. 274, Issue 19, pp. 13619-28, 1999 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).

Alternatives

Alternatives for antigen "3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1)", type "Antibodies"
Hosts Rabbit (27), Goat (4), Mouse (1)
Reactivities Human (30), Mouse (Murine) (23), Rat (Rattus) (23), Cow (Bovine) (20), Horse (Equine) (18), Rabbit (18), Sheep (Ovine) (18), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Xenopus laevis (1)
Applications Immunofluorescence (IF) (17), Western Blotting (WB) (16), ELISA (13), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes C-Term (1), N-Term (1)