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GPN1 antibody (GPN-Loop GTPase 1) (AA 9-119)

Details for Product anti-GPN1 Antibody No. ABIN394467, Supplier: Log in to see
Antigen
  • 2410004J02Rik
  • AI449615
  • ATPBD1A
  • GPN1
  • MBDIN
  • MGC137872
  • NTPBP
  • RPAP4
  • Xab1
  • XAB1
Epitope
AA 9-119
18
16
11
4
2
2
1
1
Reactivity
Human
51
7
7
Host
Mouse
35
16
Clonality (Clone)
Monoclonal ()
Conjugate
This GPN1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
44
31
11
8
8
7
5
2
Supplier
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Immunogen XAB1 (AAH07451, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3E1
Isotype IgG1 kappa
Specificity XAB1 (AAH07451, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name XAB1 (GPN1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 41740 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a guanosine triphosphatase enzyme. The encoded protein may play a role in DNA repair and may function in activation of transcription. Alternatively spliced transcript variants have been described.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-GPN1 antibody (GPN-Loop GTPase 1) (AA 9-119) (ABIN394467) Immunofluorescence of monoclonal antibody to XAB1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-GPN1 antibody (GPN-Loop GTPase 1) (AA 9-119) (ABIN394467) anti-GPN-Loop GTPase 1 (GPN1) (AA 9-119) antibody (Image 2)
Background publications Sowa, Bennett, Gygi, Harper: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Jeronimo, Forget, Bouchard, Li, Chua, Poitras, Thérien, Bergeron, Bourassa, Greenblatt, Chabot, Poirier, Hughes, Blanchette, Price, Coulombe: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Lembo, Pero, Angrisano, Vitiello, Iuliano, Bruni, Chiariotti: "MBDin, a novel MBD2-interacting protein, relieves MBD2 repression potential and reactivates transcription from methylated promoters." in: Molecular and cellular biology, Vol. 23, Issue 5, pp. 1656-65, 2003 (PubMed).