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DHOdehase (AA 32-141) antibody

Details for Product No. ABIN394474, Supplier: Log in to see
Antigen
  • CG9741
  • DHOD
  • DHODH
  • Dmel\\CG9741
  • dhod
  • l(3)s3512
  • 2810417D19Rik
  • AI834883
  • wu:fc29a01
  • zgc:103492
  • DHOdehase
  • POADS
  • URA1
Epitope
AA 32-141
15
10
10
9
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
63
17
17
10
4
3
2
1
1
Host
Mouse
58
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
59
32
17
7
4
1
1
Supplier
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Immunogen DHODH (NP_001352, 32 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6E1
Isotype IgG2a lambda
Specificity DHODH (NP_001352, 32 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DHODH
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 42867 DA
Gene ID 12406
NCBI Accession NP_001352
Research Area Cell Structure, Metabolism
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DHOdehase (AA 32-141) antibody (ABIN394474) DHODH monoclonal antibody (M01), clone 6E1 Western Blot analysis of DHODH expression ...
Immunohistochemistry (IHC) image for anti-DHOdehase (AA 32-141) antibody (ABIN394474) anti-DHOdehase (AA 32-141) antibody (Image 2)
Background publications Ng, Buckingham, Lee et al.: "Exome sequencing identifies the cause of a mendelian disorder." in: Nature genetics, Vol. 42, Issue 1, pp. 30-5, 2009 (PubMed).

Grabar, Rozman, Logar et al.: "Dihydroorotate dehydrogenase polymorphism influences the toxicity of leflunomide treatment in patients with rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 68, Issue 8, pp. 1367-8, 2009 (PubMed).

Pawlik, Herczynska, Kurzawski et al.: "The effect of exon (19C>A) dihydroorotate dehydrogenase gene polymorphism on rheumatoid arthritis treatment with leflunomide." in: Pharmacogenomics, Vol. 10, Issue 2, pp. 303-9, 2009 (PubMed).

Anney, Lasky-Su, ODúshláine et al.: "Conduct disorder and ADHD: evaluation of conduct problems as a categorical and quantitative trait in the international multicentre ADHD genetics study." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 147B, Issue 8, pp. 1369-78, 2008 (PubMed).

Walse, Dufe, Svensson et al.: "The structures of human dihydroorotate dehydrogenase with and without inhibitor reveal conformational flexibility in the inhibitor and substrate binding sites." in: Biochemistry, Vol. 47, Issue 34, pp. 8929-36, 2008 (PubMed).