DHOdehase antibody

Details for Product No. ABIN394474
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Antigen
Synonyms DHOdehase, POADS, URA1, CG9741, DHOD, DHODH, Dmel\\CG9741, dhod, l(3)s3512, wu:fc29a01, zgc:103492
Reactivity
Human
(30), (7), (7), (4), (4), (2), (2), (1)
Host
Mouse
(30), (4)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(33), (11), (10), (4), (3), (2)
Pubmed 5 references available
Catalog no. ABIN394474
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen DHODH (NP_001352, 32 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6E1
Isotype IgG2a lambda
Specificity DHODH (NP_001352, 32 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DHODH
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 42867 DA
Gene ID 12406
UniProt NP_001352
Research Area Cell Structure, Metabolism
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DHOdehase antibody DHODH monoclonal antibody (M01), clone 6E1 Western Blot analysis of DHODH expression in MCF-7 on formalin-fixed paraffin-embedded human stomach (antibody concentration 3 µg/mL).
anti-DHOdehase antibody (2) anti-DHOdehase antibody (Image 2)
Background publications Walse, Dufe, Svensson et al.: "The structures of human dihydroorotate dehydrogenase with and without inhibitor reveal conformational flexibility in the inhibitor and substrate binding sites." in: Biochemistry, Vol. 47, Issue 34, pp. 8929-36, 2008 (PubMed).

Anney, Lasky-Su, ODúshláine et al.: "Conduct disorder and ADHD: evaluation of conduct problems as a categorical and quantitative trait in the international multicentre ADHD genetics study." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 147B, Issue 8, pp. 1369-78, 2008 (PubMed).

Pawlik, Herczynska, Kurzawski et al.: "The effect of exon (19C>A) dihydroorotate dehydrogenase gene polymorphism on rheumatoid arthritis treatment with leflunomide." in: Pharmacogenomics, Vol. 10, Issue 2, pp. 303-9, 2009 (PubMed).

Grabar, Rozman, Logar et al.: "Dihydroorotate dehydrogenase polymorphism influences the toxicity of leflunomide treatment in patients with rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 68, Issue 8, pp. 1367-8, 2009 (PubMed).

Ng, Buckingham, Lee et al.: "Exome sequencing identifies the cause of a mendelian disorder." in: Nature genetics, Vol. 42, Issue 1, pp. 30-5, 2009 (PubMed).

Hosts (30), (4)
Reactivities (30), (7), (7), (4), (4), (2), (2), (1)
Applications (33), (11), (10), (4), (3), (2)
Epitopes (7), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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