UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 35-135) antibody

Details for Product No. ABIN394495
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Antigen
Synonyms MGC84436, B4Gal-T4, beta4Gal-T4, 9130402O08Rik, B4galt-IV, beta4GalT-IV, b4Gal-T4
Epitope
AA 35-135
(7), (6), (4), (2), (2), (2), (1), (1)
Reactivity
Human
(25), (5), (3)
Host
Mouse
(21), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(24), (13), (4), (2), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394495
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Immunogen B4GALT4 (NP_003769, 35 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5E2
Isotype IgG1 kappa
Specificity B4GALT4 (NP_003769, 35 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name B4GALT4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 40041 DA
Gene ID 12406
NCBI Accession NP_003769
Research Area Cell Structure, Metabolism
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose, all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 35-135) antibody Western blot analysis of B4GALT4 over-expressed 293 cell line, cotransfected with B4GALT4 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 monoclonal antibody (M01), clone 5E2. Lane 1: B4GALT4 transfected lysate(40 KDa). Lane 2: Non-transfected lysate.
anti-UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 35-135) antibody (2) anti-UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) (AA 35-135) antibody (Image 2)
Background publications Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. ..." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Fan, Yu, Zhang et al.: "Cloning and characterization of a novel member of human beta-1,4-galactosyltransferase gene family." in: Science in China. Series C, Life sciences / Chinese Academy of Sciences, Vol. 42, Issue 4, pp. 337-45, 2008 (PubMed).

Validation Images
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