UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) antibody

Antigen: UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4)

Synonyms: MGC84436

Clonality: Monoclonal (5E2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394495

Additional Information

Alternative name: B4GALT4

Gene ID: 8702

UniProt: NP_003769

Immunogen: B4GALT4 (NP_003769, 35 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 5E2

Description: Other names: B4GalT4, beta4GalT4 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: B4GALT4 (NP_003769, 35 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 40041 DA

Comments:

Background: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose, all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of B4GALT4 over-expressed 293 cell line, cotransfected with B4GALT4 Validated Chimera RNAi ( Cat # H00008702-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B4GALT4 monoclonal antibody (M01), clone 5E2 (Cat # AT1256a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of B4GALT4 expression in transfected 293T cell line by B4GALT4 monoclonal antibody (M01), clone 5E2. Lane 1: B4GALT4 transfected lysate(40 KDa). Lane 2: Non-transfected lysate.

anti-UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 4 (B4GALT4) antibody (Image 2)

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Fan, Yu, Zhang et al.: "Cloning and characterization of a novel member of human beta-1,4-galactosyltransferase gene family." in: Science in China. Series C, Life sciences / Chinese Academy of Sciences, Vol. 42, Issue 4, pp. 337-45, 2008 (PubMed).