Ras Homolog Gene Family, Member J (RHOJ) (AA 1-215) antibody

Details for Product No. ABIN394530
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Antigen
Synonyms MGC83410, RHOJ, si:dkey-100h21.1, arhj, rasl7b, tc10b, ARHJ, RASL7B, TC10B, TCL, 1110005O19Rik, AW210585, Arhj, TC10L
Epitope
AA 1-215
(7), (7), (6), (3), (1), (1)
Reactivity
Human
(23)
Host
Mouse
(12), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB)
(23), (13), (6), (6), (6), (4), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394530
450.00 $
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Immunogen RHOJ (AAH62575, 1 a.a. ~ 215 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E4
Isotype IgG1 lambda
Specificity RHOJ (AAH62575, 1 a.a. ~ 215 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RHOJ
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23821 DA
Gene ID 12406
Research Area Cancer, Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.

Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ras Homolog Gene Family, Member J (RHOJ) (AA 1-215) antibody Immunofluorescence of monoclonal antibody to RHOJ on HeLa cell (antibody concentration 60 µg/mL). RHOJ monoclonal antibody (M01), clone 1E4 Western Blot analysis of RHOJ expression in HepG2.
anti-Ras Homolog Gene Family, Member J (RHOJ) (AA 1-215) antibody (2) anti-Ras Homolog Gene Family, Member J (RHOJ) (AA 1-215) antibody (Image 2)
Background publications Ruusala, Aspenström: "Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology." in: FEBS letters, Vol. 572, Issue 1-3, pp. 159-66, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Nishikimi, Meller, Uekawa et al.: "Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes." in: FEBS letters, Vol. 579, Issue 5, pp. 1039-46, 2005 (PubMed).

Barrios-Rodiles, Brown, Ozdamar et al.: "High-throughput mapping of a dynamic signaling network in mammalian cells." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1621-5, 2005 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Validation Images
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