Ras Homolog Gene Family, Member J (RHOJ) antibody

Antigen: Ras Homolog Gene Family, Member J (RHOJ)

Synonyms: TCL, ARHJ, TC10B, RASL7B, FLJ14445, MGC34777, Arhj, TC10L, AW210585, 1110005O19Rik, MGC106003, MGC83410, RHOJ, MGC143367, si:dkey-100h21.1

Clonality: Monoclonal (1E4)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394530

Additional Information

Alternative name: RHOJ

Gene ID: 57381

Immunogen: RHOJ (AAH62575, 1 a.a. ~ 215 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 lambda

Clone: 1E4

Description: Other names: ARHJ, FLJ14445, RASL7B, TC10B, TCLras homolog gene family, member J

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RHOJ (AAH62575, 1 a.a. ~ 215 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 23821 DA

Comments:

Background: ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to RHOJ (AT3639a) on HeLa cell . [antibody concentration 60 ug/ml] RHOJ monoclonal antibody (M01), clone 1E4 Western Blot analysis of RHOJ expression in HepG2 ( Cat # L019V1 ).

anti-Ras Homolog Gene Family, Member J (RHOJ) antibody (Image 2)

Publications

Product: Ruusala, Aspenström: "Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology." in: FEBS letters, Vol. 572, Issue 1-3, pp. 159-66, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Nishikimi, Meller, Uekawa et al.: "Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes." in: FEBS letters, Vol. 579, Issue 5, pp. 1039-46, 2005 (PubMed).

Barrios-Rodiles, Brown, Ozdamar et al.: "High-throughput mapping of a dynamic signaling network in mammalian cells." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1621-5, 2005 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).