Junctophilin 1 (JPH1) antibody

Antigen: Junctophilin 1 (JPH1)

Synonyms: JP1, JP-1, DKFZp762L0313, Jp1, ENSMUSG00000054314, JPH1, jph1, bZ1M12.3, si:rp71-1m12.3

Clonality: Monoclonal (2E6)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394580

Additional Information

Alternative name: JPH1

Gene ID: 56704

UniProt: NP_065698

Immunogen: JPH1 (NP_065698, 501 a.a. ~ 580 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2E6

Description: Other names: DKFZp762L0313, JP1, JP1 junctophilin 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: JPH1 (NP_065698, 501 a.a. ~ 580 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 71686 DA

Comments:

Background: Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged JPH1 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Brandenberger, Wei, Zhang et al.: "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation." in: Nature biotechnology, Vol. 22, Issue 6, pp. 707-16, 2004 (PubMed).

Nusbaum, Mikkelsen, Zody et al.: "DNA sequence and analysis of human chromosome 8." in: Nature, Vol. 439, Issue 7074, pp. 331-5, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Yamazaki, Yamazaki, Takeshima: "New molecular components supporting ryanodine receptor-mediated Ca(2+) release: roles of junctophilin and TRIC channel in embryonic cardiomyocytes." in: Pharmacology & therapeutics, Vol. 121, Issue 3, pp. 265-72, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).