Coagulation Factor VIII (F8) antibody

Antigen: Coagulation Factor VIII (F8)

Synonyms: AHF, F8B, F8C, HEMA, FVIII, DXS1253E, F8, Cf8, Cf-8, fb61d02, wu:fb61d02

Clonality: Monoclonal (1E8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN394620

Additional Information

Alternative name: F8

Gene ID: 2157

UniProt: NP_000123

Immunogen: F8 (NP_000123, 213 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 1E8

Description: Other names: AHF, DXS1253E, F8 protein, F8B, F8C, FVIII, HEMA coagulation factor VIII, procoagulant component (hemophilia A)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: F8 (NP_000123, 213 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 267009 DA

Comments:

Background: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation, factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged F8 is approximately 3ng/ml as a capture antibody.

Publications

Product: Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Shahani, Lavendhomme, Luttun et al.: "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." in: Blood, Vol. 115, Issue 23, pp. 4902-9, 2010 (PubMed).

Hernández-Romero, Marín, Lee et al.: "Synergism between factor XII -4C>T and factor XIII Val34Leu polymorphisms in fibrinolytic therapy in acute myocardial infarction." in: Thrombosis and haemostasis, Vol. 104, Issue 3, pp. 650-2, 2010 (PubMed).

Shemirani, Pongrácz, Antalfi et al.: "Factor XIII A subunit Val34Leu polymorphism in patients suffering atherothrombotic ischemic stroke." in: Thrombosis research, Vol. 126, Issue 2, pp. 159-62, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).