Lipase, Gastric (LIPF) antibody

Antigen: Lipase, Gastric (LIPF)

Synonyms: GL, HGL, HLAL, MGC138477, MGC142271, Rnlp, RNLIP, AV082900, 2310051B21Rik, LIPF, DGL, fb73g06, zgc:56632, wu:fb73g06

Clonality: Monoclonal (2E8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394627

Additional Information

Alternative name: LIPF

Gene ID: 8513

UniProt: NP_004181

Immunogen: LIPF (NP_004181, 299 a.a. ~ 399 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2E8

Description: Other names: HGL, HLAL, MGC138477 lipase, gastric

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: LIPF (NP_004181, 299 a.a. ~ 399 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 45238 DA

Comments:

Background: This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged LIPF is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Ruaño, Bernene, Windemuth et al.: "Physiogenomic comparison of edema and BMI in patients receiving rosiglitazone or pioglitazone." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 400, Issue 1-2, pp. 48-55, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Zhang, Yang, Kim et al.: "An 8-gene signature, including methylated and down-regulated glutathione peroxidase 3, of gastric cancer." in: International journal of oncology, Vol. 36, Issue 2, pp. 405-14, 2009 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).