Vimentin (VIM) antibody

Antigen: Vimentin (VIM)

Synonyms: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Clonality: Monoclonal (3E9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394656

Additional Information

Alternative name: VIM

Gene ID: 7431

Immunogen: VIM (AAH30573, 41 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3E9

Description: Other names: FLJ36605 vimentin

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: VIM (AAH30573, 41 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 53652 DA

Comments:

Background: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to VIM (AT4513a) on HeLa cell . [antibody concentration 40 ug/ml] VIM monoclonal antibody (M01), clone 3E9 Western Blot analysis of VIM expression in HeLa ( Cat # L013V1 ).

anti-Vimentin (VIM) antibody (Image 2)

Publications

Product: Stewart, Little: "Diagnostic value and implications of vimentin expression in normal, reactive and neoplastic endocervical epithelium." in: Pathology, Vol. 42, Issue 3, pp. 217-23, 2010 (PubMed).

Lin, Broedersz, Rowat et al.: "Divalent cations crosslink vimentin intermediate filament tail domains to regulate network mechanics." in: Journal of molecular biology, Vol. 399, Issue 4, pp. 637-44, 2010 (PubMed).

Pinheiro, Bulik, Thornton et al.: "Association study of 182 candidate genes in anorexia nervosa." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 153B, Issue 5, pp. 1070-80, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).

Korita, Wakai, Ajioka et al.: "Aberrant expression of vimentin correlates with dedifferentiation and poor prognosis in patients with intrahepatic cholangiocarcinoma." in: Anticancer research, Vol. 30, Issue 6, pp. 2279-85, 2010 (PubMed).