BUD31 Homolog (S. Cerevisiae) (BUD31) (Met10) antibody

Details for Product No. ABIN394661
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Antigen
Synonyms CWC14, DDBDRAFT_0190998, DDBDRAFT_0233523, DDB_0190998, DDB_0233523, bud31, c77604, g10, C77604, Cwc14, EDG-2, EDG2, G10, YCR063W, fSAP17, Edg2, g10l, zgc:110615
Epitope
Met10
(6), (4), (2), (1), (1), (1), (1)
Reactivity
Human
(28), (14), (13), (2), (2), (1), (1), (1), (1)
Host
Mouse
(19), (9)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
(25), (13), (6), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394661
450.00 $
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Immunogen G10 (NP_003901, 35 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E10
Isotype IgG2b kappa
Specificity G10 (NP_003901, 35 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name G10
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17000 DA
Gene ID 12406
NCBI Accession NP_003901
Research Area Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-BUD31 Homolog (S. Cerevisiae) (BUD31) (Met10) antibody Detection limit for recombinant GST tagged G10 is approximately 1 ng/mL as a capture antibody.
Background publications Hla, Jackson, Appleby et al.: "Characterization of edg-2, a human homologue of the Xenopus maternal transcript G10 from endothelial cells." in: Biochimica et biophysica acta, Vol. 1260, Issue 2, pp. 227-9, 1995 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Scherer, Cheung, MacDonald et al.: "Human chromosome 7: DNA sequence and biology." in: Science (New York, N.Y.), Vol. 300, Issue 5620, pp. 767-72, 2003 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

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