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Actin-Related Protein T2 (ACTRT2) (AA 209-300) antibody

Details for Product No. ABIN394666, Supplier: Log in to see
Antigen
  • ARPM2
  • ARPT2
  • Arp-T2
  • HARPM2
  • 1700052K15Rik
  • Arpm2
Epitope
AA 209-300
5
4
3
1
1
1
1
1
Reactivity
Human
26
7
7
2
1
Host
Mouse
14
8
4
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
22
12
10
2
1
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Immunogen ACTRT2 (NP_536356, 209 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E10
Isotype IgG2a lambda
Specificity ACTRT2 (NP_536356, 209 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACTRT2 (ACTRT2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 41702 DA
Gene ID 12406
NCBI Accession NP_536356
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this intronless gene belongs to the actin family. Studies have shown that this protein may be involved in cytoskeletal organization similar to other cytoplasmic actin-related protein (ARP) subfamily members. Antibody raised against the human protein has been used to detect the protein by immunoblotting and immunofluorescence microscopy, demonstrating its specific synthesis in the testis, late in spermatid differentiation, and its localization in the calyx.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Actin-Related Protein T2 (ACTRT2) (AA 209-300) antibody (ABIN394666) ACTRT2 monoclonal antibody (M03), clone 2E10. Western Blot analysis of ACTRT2 express...
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Suzuki, Yamashita, Shirota et al.: "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions." in: Genome research, Vol. 14, Issue 9, pp. 1711-8, 2004 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Heid, Figge, Winter et al.: "Novel actin-related proteins Arp-T1 and Arp-T2 as components of the cytoskeletal calyx of the mammalian sperm head." in: Experimental cell research, Vol. 279, Issue 2, pp. 177-87, 2002 (PubMed).