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Glutaminyl-Peptide Cyclotransferase (QPCT) (AA 262-360) antibody

Details for Product No. ABIN394702, Supplier: Log in to see
Antigen
  • QPCT
  • GCT
  • QC
  • sQC
  • 5730422A13Rik
Epitope
AA 262-360
17
15
6
4
3
3
2
2
1
1
1
Reactivity
Human
56
2
1
1
1
1
1
Host
Mouse
47
9
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
5
5
2
2
2
Application
ELISA, Western Blotting (WB)
44
32
4
4
3
2
2
1
1
Supplier
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Immunogen QPCT (NP_036545, 262 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4E11
Isotype IgG2a kappa
Specificity QPCT (NP_036545, 262 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name QPCT (QPCT Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 40877 DA
Gene ID 12406
NCBI Accession NP_036545
Research Area Cell Structure
Pathways
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Glutaminyl-Peptide Cyclotransferase (QPCT) (AA 262-360) antibody (ABIN394702) Detection limit for recombinant GST tagged QPCT is approximately 0.03 ng/mL as a capt...
Background publications Marroni, Pfeufer, Aulchenko et al.: "A genome-wide association scan of RR and QT interval duration in 3 European genetically isolated populations: the EUROSPAN project." in: Circulation. Cardiovascular genetics, Vol. 2, Issue 4, pp. 322-8, 2009 (PubMed).

Stephan, Wermann, von Bohlen et al.: "Mammalian glutaminyl cyclases and their isoenzymes have identical enzymatic characteristics." in: The FEBS journal, Vol. 276, Issue 22, pp. 6522-36, 2010 (PubMed).

Cynis, Rahfeld, Stephan et al.: "Isolation of an isoenzyme of human glutaminyl cyclase: retention in the Golgi complex suggests involvement in the protein maturation machinery." in: Journal of molecular biology, Vol. 379, Issue 5, pp. 966-80, 2008 (PubMed).

Calvaresi, Garavelli, Bottoni: "Computational evidence for the catalytic mechanism of glutaminyl cyclase. A DFT investigation." in: Proteins, Vol. 73, Issue 3, pp. 527-38, 2008 (PubMed).

Huang, Wang, Chang et al.: "A conserved hydrogen-bond network in the catalytic centre of animal glutaminyl cyclases is critical for catalysis." in: The Biochemical journal, Vol. 411, Issue 1, pp. 181-90, 2008 (PubMed).