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AFF4 antibody (AF4/FMR2 Family, Member 4) (AA 1-109)

Details for Product anti-AFF4 Antibody No. ABIN394716, Supplier: Login to see New
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Antigen
Synonyms AFF4, RA_m002_jsmFBA6Br, AF5Q31, MCEF, Alf4, Laf4l
Epitope
AA 1-109
(19), (18), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(58), (22), (20)
Host
Mouse
(52), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
This AFF4 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(41), (24), (11), (4), (4), (2), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
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Immunogen AFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E12
Isotype IgG1 kappa
Specificity AFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AFF4 (AFF4 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 127459 DA
Gene ID 12406
NCBI Accession NP_055238
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AFF4 antibody (AF4/FMR2 Family, Member 4) (AA 1-109) (ABIN394716) Western Blot analysis of AFF4 expression in transfected 293T cell line by AFF4 monocl...
Immunohistochemistry (IHC) image for anti-AFF4 antibody (AF4/FMR2 Family, Member 4) (AA 1-109) (ABIN394716) anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-109) antibody (Image 2)
Background publications He, Liu, Hsu et al.: "HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription." in: Molecular cell, Vol. 38, Issue 3, pp. 428-38, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Lin, Smith, Takahashi et al.: "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia." in: Molecular cell, Vol. 37, Issue 3, pp. 429-37, 2010 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Niedzielski, Hopewell, Ismail et al.: "MCEF is localized to the nucleus by protein sequences encoded within three distinct exons, where it represses HIV-1 Tat-transactivation of LTR-directed transcription." in: International journal of biological sciences, Vol. 3, Issue 4, pp. 225-36, 2007 (PubMed).

Catalog No. ABIN394716
$450.00
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