anti-AFF4 antibody (AF4/FMR2 Family, Member 4) (AA 1-109)

Details for Product anti-AFF4 Antibody No. ABIN394716
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Synonyms AFF4, RA_m002_jsmFBA6Br, AF5Q31, MCEF, Alf4, Laf4l
AA 1-109
(17), (17), (4), (2), (2), (2), (2), (1), (1), (1), (1)
(53), (19), (17)
(47), (7)
Clonality (Clone)
Monoclonal ()
This AFF4 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(39), (21), (10), (4), (4), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 8 to 11 Business Days
Immunogen AFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E12
Isotype IgG1 kappa
Specificity AFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AFF4 (AFF4 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 127459 DA
Gene ID 12406
NCBI Accession NP_055238
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AFF4 antibody (AF4/FMR2 Family, Member 4) (AA 1-109) (ABIN394716) Western Blot analysis of AFF4 expression in transfected 293T cell line by AFF4 monocl...
Immunohistochemistry (IHC) image for anti-AFF4 antibody (AF4/FMR2 Family, Member 4) (AA 1-109) (ABIN394716) anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-109) antibody (Image 2)
Background publications He, Liu, Hsu et al.: "HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription." in: Molecular cell, Vol. 38, Issue 3, pp. 428-38, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Lin, Smith, Takahashi et al.: "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia." in: Molecular cell, Vol. 37, Issue 3, pp. 429-37, 2010 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Niedzielski, Hopewell, Ismail et al.: "MCEF is localized to the nucleus by protein sequences encoded within three distinct exons, where it represses HIV-1 Tat-transactivation of LTR-directed transcription." in: International journal of biological sciences, Vol. 3, Issue 4, pp. 225-36, 2007 (PubMed).

Catalog No. ABIN394716
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