AF4/FMR2 Family, Member 4 (AFF4) antibody

Details for Product No. ABIN394716
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms AF5Q31, MCEF, Alf4, Laf4l, AFF4, RA_m002_jsmFBA6Br
Reactivity
Human
(34), (16), (15), (11), (11), (11)
Host
Mouse
(31), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(21), (15), (9), (3), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN394716
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen AFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E12
Isotype IgG1 kappa
Specificity AFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AFF4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 127459 DA
Gene ID 12406
UniProt NP_055238
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-AF4/FMR2 Family, Member 4 (AFF4) antibody Western Blot analysis of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody (M01), clone 2E12. Lane 1: AFF4 transfected lysate( 40.2 KDa). Lane 2: Non-transfected lysate. Immunoperoxidase of monoclonal antibody to AFF4 on formalin-fixed paraffin-embedded human endometrium (antibody concentration 1 µg/mL).
anti-AF4/FMR2 Family, Member 4 (AFF4) antibody (2) anti-AF4/FMR2 Family, Member 4 (AFF4) antibody (Image 2)
Background publications Niedzielski, Hopewell, Ismail et al.: "MCEF is localized to the nucleus by protein sequences encoded within three distinct exons, where it represses HIV-1 Tat-transactivation of LTR-directed transcription." in: International journal of biological sciences, Vol. 3, Issue 4, pp. 225-36, 2007 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Lin, Smith, Takahashi et al.: "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia." in: Molecular cell, Vol. 37, Issue 3, pp. 429-37, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

He, Liu, Hsu et al.: "HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription." in: Molecular cell, Vol. 38, Issue 3, pp. 428-38, 2010 (PubMed).

Hosts (31), (4)
Reactivities (34), (16), (15), (11), (11), (11)
Applications (21), (15), (9), (3), (2), (2), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (10), (9), (2), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top