Ras Homolog Gene Family, Member C (RHOC) antibody

Antigen: Ras Homolog Gene Family, Member C (RHOC)

Synonyms: H9, ARH9, ARHC, RHOH9, MGC1448, MGC61427, Arh9, Arhc, AI324259, MGC52893, MGC132110, RHOC, CRHOC, MGC140228, DKFZp469H2018, LOC100221682

Clonality: Monoclonal (2E12)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394719

Additional Information

Alternative name: RHOC

Gene ID: 389

Immunogen: RHOC (AAH07245, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2E12

Description: Other names: ARH9, ARHC, RHOH9 ras homolog gene family, member C

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RHOC (AAH07245, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 22006 DA

Comments:

Background: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western Blot analysis of RHOC expression in transfected 293T cell line by RHOC monoclonal antibody (M01), clone 2E12. Lane 1: RHOC transfected lysate(22 KDa). Lane 2: Non-transfected lysate. RHOC monoclonal antibody (M01), clone 2E12 Western Blot analysis of RHOC expression in Jurkat ( Cat # L017V1 ).

anti-Ras Homolog Gene Family, Member C (RHOC) antibody (Image 2)

Publications

Product: Segat, Brandão, Guimarães et al.: "Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment." in: Vaccine, Vol. 28, Issue 10, pp. 2201-6, 2010 (PubMed).

Kitzing, Wang, Pertz et al.: "Formin-like 2 drives amoeboid invasive cell motility downstream of RhoC." in: Oncogene, Vol. 29, Issue 16, pp. 2441-8, 2010 (PubMed).

Jiang, Liu, Kolokythas et al.: "Downregulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 127, Issue 3, pp. 505-12, 2010 (PubMed).

Lipkin, Chao, Moreno et al.: "Genetic variation in 3-hydroxy-3-methylglutaryl CoA reductase modifies the chemopreventive activity of statins for colorectal cancer." in: Cancer prevention research (Philadelphia, Pa.), Vol. 3, Issue 5, pp. 597-603, 2010 (PubMed).

Wu, Wu, Rosenthal et al.: "Characterization of the roles of RHOC and RHOA GTPases in invasion, motility, and matrix adhesion in inflammatory and aggressive breast cancers." in: Cancer, Vol. 116, Issue 11 Suppl, pp. 2768-82, 2010 (PubMed).