You are viewing an incomplete version of our website. Please click to reload the website as full version.

BCL2-Associated Athanogene 2 (BAG2) (AA 102-212) antibody

Details for Product No. ABIN394729, Supplier: Login to see
Antigen
  • bag2
  • MGC89300
  • BAG2
  • ATBAG2
  • BCL-2-associated athanogene 2
  • MTG10.13
  • MTG10_13
  • BAG-2
  • dJ417I1.2
  • zgc:64195
  • 2610042A13Rik
  • BC016230
Epitope
AA 102-212
35
13
9
8
5
4
2
2
2
2
2
1
1
Reactivity
Human
76
22
21
4
3
2
2
1
1
1
1
1
Host
Mouse
54
16
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
59
33
22
11
10
6
4
4
1
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen BAG2 (NP_004273, 102 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6E12
Isotype IgG1 kappa
Specificity BAG2 (NP_004273, 102 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BAG2 (BAG2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23772 DA
Gene ID 12406
NCBI Accession NP_004273
Research Area Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BCL2-Associated Athanogene 2 (BAG2) (AA 102-212) antibody (ABIN394729) BAG2 monoclonal antibody (M01), clone 6E12 Western Blot analysis of BAG2 expression i...
Background publications Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Arndt, Daniel, Nastainczyk et al.: "BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP." in: Molecular biology of the cell, Vol. 16, Issue 12, pp. 5891-900, 2005 (PubMed).

Dai, Qian, Li et al.: "Regulation of the cytoplasmic quality control protein degradation pathway by BAG2." in: The Journal of biological chemistry, Vol. 280, Issue 46, pp. 38673-81, 2005 (PubMed).