BCL2-Associated Athanogene 2 (BAG2) (AA 102-212) antibody

Details for Product No. ABIN394729
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Antigen
Synonyms bag2, MGC89300, BAG2, ATBAG2, BCL-2-associated athanogene 2, MTG10.13, MTG10_13, BAG-2, dJ417I1.2, zgc:64195, 2610042A13Rik, BC016230
Epitope
AA 102-212
(14), (7), (2), (2), (2), (1), (1)
Reactivity
Human
(40), (17), (16), (14), (13), (12), (2), (1), (1), (1)
Host
Mouse
(34), (5), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(29), (13), (12), (10), (6), (4), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394729
450.00 $
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  • +1 404 474 4654
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Immunogen BAG2 (NP_004273, 102 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6E12
Isotype IgG1 kappa
Specificity BAG2 (NP_004273, 102 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BAG2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23772 DA
Gene ID 12406
NCBI Accession NP_004273
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-BCL2-Associated Athanogene 2 (BAG2) (AA 102-212) antibody BAG2 monoclonal antibody (M01), clone 6E12 Western Blot analysis of BAG2 expression in HeLa.
Background publications Dai, Qian, Li et al.: "Regulation of the cytoplasmic quality control protein degradation pathway by BAG2." in: The Journal of biological chemistry, Vol. 280, Issue 46, pp. 38673-81, 2005 (PubMed).

Arndt, Daniel, Nastainczyk et al.: "BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP." in: Molecular biology of the cell, Vol. 16, Issue 12, pp. 5891-900, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

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