BCL2-Associated Athanogene 2 (BAG2) antibody

Antigen: BCL2-Associated Athanogene 2 (BAG2)

Synonyms: BAG-2, KIAA0576, MGC149462, dJ417I1.2, BC016230, MGC36682, 2610042A13Rik, MGC64195, zgc:64195, bag2, MGC89300, BAG2, MGC187837, MGC128966

Clonality: Monoclonal (6E12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394729

Additional Information

Alternative name: BAG2

Gene ID: 9532

UniProt: NP_004273

Immunogen: BAG2 (NP_004273, 102 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 6E12

Description: Other names: BAG2, KIAA0576, dJ417I1.2 BCL2-associated athanogene 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BAG2 (NP_004273, 102 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 23772 DA

Comments:

Background: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

BAG2 monoclonal antibody (M01), clone 6E12 Western Blot analysis of BAG2 expression in HeLa ( Cat # L013V1 ).

Publications

Product: Dai, Qian, Li et al.: "Regulation of the cytoplasmic quality control protein degradation pathway by BAG2." in: The Journal of biological chemistry, Vol. 280, Issue 46, pp. 38673-81, 2005 (PubMed).

Arndt, Daniel, Nastainczyk et al.: "BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP." in: Molecular biology of the cell, Vol. 16, Issue 12, pp. 5891-900, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).