Mesothelin (MSLN) antibody

Antigen: Mesothelin (MSLN)

Synonyms: MPF, SMR, CAK1, MSLN

Clonality: Monoclonal (1A10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394740

Additional Information

Alternative name: MSLN

Gene ID: 10232

UniProt: NP_005814

Immunogen: MSLN (NP_005814, 464 a.a. ~ 564 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 1A10

Description: Other names: CAK1, MPF, SMR mesothelin

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MSLN (NP_005814, 464 a.a. ~ 564 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 68986 DA

Comments:

Background: This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged MSLN is approximately 1ng/ml as a capture antibody.

Publications

Product: Cristaudo, Foddis, Bonotti et al.: "Polymorphisms in the putative micro-RNA-binding sites of mesothelin gene are associated with serum levels of mesothelin-related protein." in: Occupational and environmental medicine, Vol. 67, Issue 4, pp. 233-6, 2010 (PubMed).

Rai, Flores, Mathew et al.: "Soluble mesothelin related peptides (SMRP) and osteopontin as protein biomarkers for malignant mesothelioma: analytical validation of ELISA based assays and characterization at mRNA and protein levels." in: Clinical chemistry and laboratory medicine : CCLM / FESCC, Vol. 48, Issue 2, pp. 271-8, 2010 (PubMed).

Obulhasim, Fujii, Matsumoto et al.: "Mesothelin gene expression and promoter methylation/hypomethylation in gynecological tumors." in: European journal of gynaecological oncology, Vol. 31, Issue 1, pp. 63-71, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Tan, Kajino, Momose et al.: "Mesothelin (MSLN) promoter is hypomethylated in malignant mesothelioma, but its expression is not associated with methylation status of the promoter." in: Human pathology, Vol. 41, Issue 9, pp. 1330-8, 2010 (PubMed).