DDB1 and CUL4 Associated Factor 8 (DCAF8) antibody

Antigen: DDB1 and CUL4 Associated Factor 8 (DCAF8)

Synonyms: H326, WDR42A, FLJ35857, MGC99640, MGC117276, MGC118891, DKFZp781G1096, C76055, Wdr42a, D1Ucla4, AA408877, AA433120, AA792214, AU018973, D1Dau35e, MGC38511, RGD1308513, DCAF8, MGC157366, wdr42a, DKFZp459M072

Clonality: Monoclonal (1A12)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394755

Additional Information

Alternative name: WDR42A

Gene ID: 50717

Immunogen: WDR42A (AAH29399, 1 a.a. ~ 274 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgM kappa

Clone: 1A12

Description: Other names: DKFZp781G1096, H326, MGC99640 WD repeat domain 42A

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WDR42A (AAH29399, 1 a.a. ~ 274 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 66852 DA

Comments:

Background: This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene.

Synonyms: H326, WDR42A, FLJ35857, MGC99640, MGC117276, MGC118891, DKFZp781G1096, C76055, Wdr42a, D1Ucla4, AA408877, AA433120, AA792214, AU018973, D1Dau35e, MGC38511, RGD1308513, DCAF8, MGC157366, wdr42a, DKFZp459M072

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

WDR42A monoclonal antibody (M01A), clone 1A12 Western Blot analysis of WDR42A expression in A-549 ( Cat # L025V1 ).

Publications

Product: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Jin, Arias, Chen et al.: "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." in: Molecular cell, Vol. 23, Issue 5, pp. 709-21, 2006 (PubMed).

Angers, Li, Yi et al.: "Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery." in: Nature, Vol. 443, Issue 7111, pp. 590-3, 2006 (PubMed).

Venkatesan, Rual, Vazquez et al.: "An empirical framework for binary interactome mapping." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).