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AHA1, Activator of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast) (AHSA1) (AA 1-339) antibody

Details for Product No. ABIN394761, Supplier: Log in to see
Antigen
  • AHA1
  • ahsa1
  • AHSA1
  • BC023857
  • C14orf3
  • MGC52884
  • MGC80312
  • MGC89589
  • MGC132147
  • p38
  • wu:fb83b05
  • zgc:56075
  • zgc:136821
Epitope
AA 1-339
10
8
6
6
4
4
4
3
2
2
1
1
1
1
Reactivity
Human
189
163
74
4
1
Host
Mouse
116
92
48
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
12
12
10
8
8
8
8
8
8
8
8
8
8
8
8
8
8
Application
Western Blotting (WB)
245
213
139
136
118
113
17
6
4
3
1
Supplier
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Immunogen AHSA1 (AAH00321, 1 a.a. ~ 339 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A2-A8
Isotype IgG1 kappa
Specificity AHSA1 (AAH00321, 1 a.a. ~ 339 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AHSA1 (AHSA1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 38274 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AHA1, Activator of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast) (AHSA1) (AA 1-339) antibody (ABIN394761) AHSA1 monoclonal antibody (M01), clone 1A2-A8 Western Blot analysis of AHSA1 expressi...
Background publications Mollapour, Tsutsumi, Donnelly, Beebe, Tokita, Lee, Lee, Morra, Bourboulia, Scroggins, Colombo, Blagg, Panaretou, Stetler-Stevenson, Trepel, Piper, Prodromou, Pearl, Neckers: "Swe1Wee1-dependent tyrosine phosphorylation of Hsp90 regulates distinct facets of chaperone function." in: Molecular cell, Vol. 37, Issue 3, pp. 333-43, 2010

Koulov, Lapointe, Lu, Razvi, Coppinger, Dong, Matteson, Laister, Arrowsmith, Yates, Balch: "Biological and structural basis for Aha1 regulation of Hsp90 ATPase activity in maintaining proteostasis in the human disease cystic fibrosis." in: Molecular biology of the cell, Vol. 21, Issue 6, pp. 871-84, 2010

Barbe, Lundberg, Oksvold, Stenius, Lewin, Björling, Asplund, Pontén, Brismar, Uhlén, Andersson-Svahn: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007

Wang, Venable, LaPointe, Hutt, Koulov, Coppinger, Gurkan, Kellner, Matteson, Plutner, Riordan, Kelly, Yates, Balch: "Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis." in: Cell, Vol. 127, Issue 4, pp. 803-15, 2006