UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1) (AA 111-211) antibody

Details for Product No. ABIN394787
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Antigen
Synonyms B3gnt6, B3GN-T1, B3GNT6, BETA3GNTI, MDDGA13, iGAT, iGNT, 1500032M01Rik, BETA3GNT1, B3GNT2
Epitope
AA 111-211
(8), (7), (2), (2), (1), (1), (1)
Reactivity
Human
(14), (1)
Host
Mouse
(11), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(13), (8), (7), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394787
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Immunogen B3GNT1 (NP_006568, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1A8
Isotype IgG2a kappa
Specificity B3GNT1 (NP_006568, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name B3GNT1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 46022 DA
Gene ID 12406
NCBI Accession NP_006568
Research Area Cell Structure, Metabolism
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1) (AA 111-211) antibody Western Blot analysis of B3GNT1 expression in transfected 293T cell line by B3GNT1 monoclonal antibody (M05), clone 1A8. Lane 1: B3GNT1 transfected lysate(46.022 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged B3GNT1 is approximately 0.3 ng/mL as a capture antibody.
anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1) (AA 111-211) antibody (2) anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1) (AA 111-211) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Zheng, Li, Ji et al.: "Characterization of a cDNA encoding a protein with limited similarity to beta1, 3-N-acetylglucosaminyltransferase." in: Molecular biology reports, Vol. 31, Issue 3, pp. 171-5, 2004 (PubMed).

Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Seko, Yamashita: "Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33094-100, 2008 (PubMed).

, Reveille, Sims et al.: "Genome-wide association study of ankylosing spondylitis identifies non-MHC susceptibility loci." in: Nature genetics, Vol. 42, Issue 2, pp. 123-7, 2010 (PubMed).

Validation Images
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