UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1) antibody

Antigen: UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1)

Synonyms: B3GNT, B3GNT1, B3GN-T1, B3GN-T2, B3GNT-2, BETA3GNT, iGAT, iGNT, B3GNT6, BETA3GNTI, BGnT1, BGnT2, B3gnt1, BGnT-1, BGnT-2, B3Galt6, AA408337, AA409404, AW260308, Beta3gnt, B3gnt6, BETA3GNT1, 1500032M01Rik, B3GNT2, MGC128466, DKFZp459K1316

Clonality: Monoclonal (1A8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394787

Additional Information

Alternative name: B3GNT1

Gene ID: 10678

UniProt: NP_006568

Immunogen: B3GNT1 (NP_006568, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1A8

Description: Other names: B3GNT1, B3GNT2, B3GNT, B3GNT2, BETA3GNT UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: B3GNT1 (NP_006568, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 46022 DA

Comments:

Background: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains.

Synonyms: B3GNT, B3GNT1, B3GN-T1, B3GN-T2, B3GNT-2, BETA3GNT, iGAT, iGNT, B3GNT6, BETA3GNTI, BGnT1, BGnT2, B3gnt1, BGnT-1, BGnT-2, B3Galt6, AA408337, AA409404, AW260308, Beta3gnt, B3gnt6, BETA3GNT1, 1500032M01Rik, B3GNT2, MGC128466, DKFZp459K1316

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Metabolism

Restrictions: For Research Use only

Images

Western Blot analysis of B3GNT1 expression in transfected 293T cell line by B3GNT1 monoclonal antibody (M05), clone 1A8. Lane 1: B3GNT1 transfected lysate(46.022 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged B3GNT1 is approximately 0.3ng/ml as a capture antibody.

anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Zheng, Li, Ji et al.: "Characterization of a cDNA encoding a protein with limited similarity to beta1, 3-N-acetylglucosaminyltransferase." in: Molecular biology reports, Vol. 31, Issue 3, pp. 171-5, 2004 (PubMed).

Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Seko, Yamashita: "Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33094-100, 2008 (PubMed).

, Reveille, Sims et al.: "Genome-wide association study of ankylosing spondylitis identifies non-MHC susceptibility loci." in: Nature genetics, Vol. 42, Issue 2, pp. 123-7, 2010 (PubMed).