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Acylphosphatase 1, Erythrocyte (Common) Type (Acyp1) antibody

Details for Product No. ABIN394834
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Antigen
Synonyms CH2, ACYPE, AI325944, 1110039O14Rik, ACYP1, eap1, im:7149755, wu:fj62c08, zgc:163122
Reactivity
Human
(23), (7), (7)
Host
Mouse
(13), (8), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (18), (4), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394834
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen ACYP1 (AAH35568, 1 a.a. ~ 100 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1B2-3A2
Isotype IgG2b kappa
Specificity ACYP1 (AAH35568, 1 a.a. ~ 100 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACYP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 11261 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Acylphosphatase 1, Erythrocyte (Common) Type (Acyp1) antibody Western Blot analysis of ACYP1 expression in transfected 293T cell line by ACYP1 monoclonal antibody (M01), clone 1B2-3A2. Lane 1: ACYP1 transfected lysate(11.3 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged ACYP1 is approximately 0.03 ng/mL as a capture antibody.
anti-Acylphosphatase 1, Erythrocyte (Common) Type (Acyp1) antibody (2) anti-Acylphosphatase 1, Erythrocyte (Common) Type (Acyp1) antibody (Image 2)
Background publications Paoli, Pazzagli, Giannoni et al.: "A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 194-9, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Heilig, Eckenberg, Petit et al.: "The DNA sequence and analysis of human chromosome 14...." in: Nature, Vol. 421, Issue 6923, pp. 601-7, 2003 (PubMed).

DeglInnocenti, Marzocchini, Malentacchi et al.: "ACYP1 gene possesses two alternative splicing forms that induce apoptosis." in: IUBMB life, Vol. 56, Issue 1, pp. 29-33, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN394834
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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