Breast Carcinoma Amplified Sequence 1 (BCAS1) antibody

Antigen: Breast Carcinoma Amplified Sequence 1 (BCAS1)

Synonyms: AIBC1, NABC1, AI841227, 2210416M21Rik, 9030223A09Rik, Band83, Pmes-2, BCAS1, MGC152145

Clonality: Monoclonal (1B5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394844

Additional Information

Alternative name: BCAS1

Gene ID: 8537

UniProt: NP_003648

Immunogen: BCAS1 (NP_003648, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1B5

Description: Other names: AIBC1, NABC1breast carcinoma amplified sequence 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BCAS1 (NP_003648, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 61709 DA

Comments:

Background: This gene resides in a region at 20q13 which is amplified in a variety of tumor types and associated with more aggressive tumor phenotypes. Among the genes identified from this region, it was found to be highly expressed in three amplified breast cancer cell lines and in one breast tumor without amplification at 20q13.2. However, this gene is not in the common region of maximal amplification and its expression was not detected in the breast cancer cell line MCF7, in which this region is highly amplified. Although not consistently expressed, this gene is a candidate oncogene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to BCAS1 ( Cat# AT1279a ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml]

Publications

Product: Beardsley, Kowbel, Lataxes et al.: "Characterization of the novel amplified in breast cancer-1 (NABC1) gene product." in: Experimental cell research, Vol. 290, Issue 2, pp. 402-13, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Burmester, Suarez, Lin et al.: "Analysis of candidate genes for prostate cancer." in: Human heredity, Vol. 57, Issue 4, pp. 172-8, 2004 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).