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Adaptor-Related Protein Complex 4, beta 1 Subunit (AP4B1) (AA 640-740) antibody

Details for Product No. ABIN394856
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Antigen
Synonyms AP4B1, DDBDRAFT_0185456, DDBDRAFT_0234245, DDB_0185456, DDB_0234245, BETA-4, CPSQ5, SPG47, 1810038H16Rik, AV004952, Ap4b4
Epitope
AA 640-740
(7), (6), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(16)
Host
Mouse
(11), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(14), (10), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN394856
450.00 $
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Immunogen AP4B1 (NP_006585, 640 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1B7
Isotype IgG1 kappa
Specificity AP4B1 (NP_006585, 640 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AP4B1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 83260 DA
Gene ID 12406
UniProt NP_006585
Research Area Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The heterotetrameric adaptor protein (AP) complexes sort integral membrane proteins at various stages of the endocytic and secretory pathways. AP4 is composed of 2 large chains, beta-4 (AP4B1) and epsilon-4 (AP4E1, MIM 607244), a medium chain, mu-4 (AP4M1, MIM 602296), and a small chain, sigma-4 (AP4S1, MIM 607243).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Adaptor-Related Protein Complex 4, beta 1 Subunit (AP4B1) (AA 640-740) antibody Detection limit for recombinant GST tagged AP4B1 is approximately 0.3 ng/mL as a capture antibody.
Background publications Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

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