Adaptor-Related Protein Complex 4, beta 1 Subunit (AP4B1) antibody

Antigen: Adaptor-Related Protein Complex 4, beta 1 Subunit (AP4B1)

Synonyms: BETA-4, AP4B1, MGC155066, Ap4b4, AV004952, 1810038H16Rik

Clonality: Monoclonal (1B7)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394856

Additional Information

Alternative name: AP4B1

Gene ID: 10717

UniProt: NP_006585

Immunogen: AP4B1 (NP_006585, 640 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1B7

Description: Other names: BETA4 adaptor-related protein complex 4, beta 1 subunit

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AP4B1 (NP_006585, 640 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 83260 DA

Comments:

Background: The heterotetrameric adaptor protein (AP) complexes sort integral membrane proteins at various stages of the endocytic and secretory pathways. AP4 is composed of 2 large chains, beta-4 (AP4B1) and epsilon-4 (AP4E1, MIM 607244), a medium chain, mu-4 (AP4M1, MIM 602296), and a small chain, sigma-4 (AP4S1, MIM 607243).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged AP4B1 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).