F2 antibody

Antigen: F2

Clonality: Monoclonal (1B7)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394859

Additional Information

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Gene ID: 2147

Immunogen: F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG Mix kappa  (Matching secondary antibodies)

Clone: 1B7

Description: Other names: PT coagulation factor II (thrombin)

Molecular Weight: 70037 DA

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7. Lane 1: F2 transfected lysate(70 KDa). Lane 2: Non-transfected lysate. Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # AT1977a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-F2 antibody (Image 2)