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Src Kinase Associated phosphoprotein 1 (SKAP1) antibody
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||SCAP1 (NP_003717, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: SKAP55 src family associated phosphoprotein 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||SCAP1 (NP_003717, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||41462 DA|
Background: This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Signaling, Cell Structure|
|Restrictions||For Research Use only|
Kosco, Cerignoli, Williams et al.: "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1." in: Molecular immunology, Vol. 45, Issue 2, pp. 510-22, 2007 (PubMed).
Fang, Seki, Fang: "SKAP associates with kinetochores and promotes the metaphase-to-anaphase transition." in: Cell cycle (Georgetown, Tex.), Vol. 8, Issue 17, pp. 2819-27, 2009 (PubMed).
Lehmann, Meyer, Schuemann et al.: "A novel S3S-TAP-tag for the isolation of T-cell interaction partners of adhesion and degranulation promoting adaptor protein." in: Proteomics, Vol. 9, Issue 23, pp. 5288-95, 2009 (PubMed).
Raab, Wang, Lu et al.: "T cell receptor "inside-out" pathway via signaling module SKAP1-RapL regulates T cell motility and interactions in lymph nodes." in: Immunity, Vol. 32, Issue 4, pp. 541-56, 2010 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).
|Hosts||Rabbit (9), Mouse (2), Goat (1)|
|Reactivities||Human (11), Mouse (Murine) (1), Rat (Rattus) (1)|
|Applications||Western Blotting (WB) (12), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1)|
|Epitopes||AA 1-358 (1), C-Term,AA 265-295 (1), N-Term (1)|