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Apoptosis Inhibitor 5 antibody (API5) (AA 400-504)

Details for Product anti-API5 Antibody No. ABIN394905, Supplier: Log in to see
Antigen
  • api5l1
  • sr:nyz021
  • zgc:66211
  • wu:fb26c03
  • api5
  • API-5A
  • api5-A
  • MGC83290
  • API5
  • AAC-11
  • AAC11
  • AI196452
Epitope
AA 400-504
34
14
13
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
70
31
25
2
Host
Mouse
67
3
Clonality (Clone)
Monoclonal ()
Conjugate
This Apoptosis Inhibitor 5 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
49
30
16
13
11
8
5
3
2
1
Supplier
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Immunogen API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C2
Isotype IgG2b kappa
Specificity API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name API5 (API5 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 57561 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Apoptosis Inhibitor 5 antibody (API5) (AA 400-504) (ABIN394905) Immunofluorescence of monoclonal antibody to API5 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-Apoptosis Inhibitor 5 antibody (API5) (AA 400-504) (ABIN394905) anti-Apoptosis Inhibitor 5 (API5) (AA 400-504) antibody (Image 2)
Background publications Ren, Zhang, Shi et al.: "Pim-2 activates API-5 to inhibit the apoptosis of hepatocellular carcinoma cells through NF-kappaB pathway." in: Pathology oncology research : POR, Vol. 16, Issue 2, pp. 229-37, 2010 (PubMed).

Need, Attix, McEvoy et al.: "A genome-wide study of common SNPs and CNVs in cognitive performance in the CANTAB." in: Human molecular genetics, Vol. 18, Issue 23, pp. 4650-61, 2009 (PubMed).

Rigou, Piddubnyak, Faye et al.: "The antiapoptotic protein AAC-11 interacts with and regulates Acinus-mediated DNA fragmentation." in: The EMBO journal, Vol. 28, Issue 11, pp. 1576-88, 2009 (PubMed).

Wang, Lee, Ma et al.: "Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 13205-15, 2008 (PubMed).

Morris, Michaud, Ji et al.: "Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo." in: PLoS genetics, Vol. 2, Issue 11, pp. e196, 2006 (PubMed).