ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2) antibody

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2)

Synonyms: DJS, MRP2, cMRP, ABC30, CMOAT, KIAA1010

Clonality: Monoclonal (1C5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN394929

Additional Information

Alternative name: ABCC2

Gene ID: 1244

UniProt: NP_000383

Immunogen: ABCC2 (NP_000383, 214 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1C5

Description: Other names: ABC30, CMOAT, DJS, KIAA1010, MRP2, cMRP ATP-binding cassette, sub-family C (CFTR/MRP), member 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ABCC2 (NP_000383, 214 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 174191 DA

Comments:

Background: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine, therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ABCC2 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

McLeod, Sargent, Marsh et al.: "Pharmacogenetic predictors of adverse events and response to chemotherapy in metastatic colorectal cancer: results from North American Gastrointestinal Intergroup Trial N9741." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 20, pp. 3227-33, 2010 (PubMed).

Woillard, Rerolle, Picard et al.: "Risk of diarrhoea in a long-term cohort of renal transplant patients given mycophenolate mofetil: the significant role of the UGT1A8 2 variant allele." in: British journal of clinical pharmacology, Vol. 69, Issue 6, pp. 675-83, 2010 (PubMed).

Hu, Lui, Mak et al.: "Pharmacogenetic analysis of lipid responses to rosuvastatin in Chinese patients." in: Pharmacogenetics and genomics, Vol. 20, Issue 10, pp. 634-7, 2010 (PubMed).

Takahashi, Miura, Scott et al.: "Influence of CYP3A5 and drug transporter polymorphisms on imatinib trough concentration and clinical response among patients with chronic phase chronic myeloid leukemia." in: Journal of human genetics, Vol. 55, Issue 11, pp. 731-7, 2010 (PubMed).