Epstein-Barr Virus Induced 3 (EBI3) antibody

Antigen: Epstein-Barr Virus Induced 3 (EBI3)

Clonality: Monoclonal (1D3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN394997

Additional Information

Alternative name: EBI3

Gene ID: 10148

UniProt: NP_005746

Immunogen: EBI3 (NP_005746, 129 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1D3

Description: Other names: Epstein-Barr virus induced gene 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EBI3 (NP_005746, 129 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 25396 DA

Comments:

Background: This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged EBI3 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Krumbiegel, Anthogalidis-Voss, Markus et al.: "Enhanced expression of IL-27 mRNA in human newborns." in: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology, Vol. 19, Issue 6, pp. 513-6, 2008 (PubMed).

Poleganov, Bachmann, Pfeilschifter et al.: "Genome-wide analysis displays marked induction of EBI3/IL-27B in IL-18-activated AML-derived KG1 cells: critical role of two kappaB binding sites in the human EBI3 promotor." in: Molecular immunology, Vol. 45, Issue 10, pp. 2869-80, 2008 (PubMed).

Carl, Bai: "IL27: its roles in the induction and inhibition of inflammation." in: International journal of clinical and experimental pathology, Vol. 1, Issue 2, pp. 117-23, 2008 (PubMed).

Kempe, Heinz, Kokai et al.: "Epstein-barr virus-induced gene-3 is expressed in human atheroma plaques." in: The American journal of pathology, Vol. 175, Issue 1, pp. 440-7, 2009 (PubMed).