Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) antibody

Details for Product No. ABIN395003
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Antigen
Synonyms cstf3a, CSTF-77, 4732468G05Rik, C79532, CstF-77, zgc:56028, wu:fa99c02, wu:fb18b09, cstf3, MGC76035, CSTF3
Reactivity
Human
(24), (15), (15), (14), (14), (14), (2), (2), (1)
Host
Mouse
(21), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(17), (10), (7), (5), (2), (1)
Pubmed 5 references available
Catalog no. ABIN395003
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D4
Isotype IgG1 kappa
Specificity CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CSTF3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 82922 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) antibody Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell (antibody concentration 10 µg/mL). Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi used as specificity and loading control.
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) antibody (2) anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) antibody (Image 2)
Background publications Kim, Bhinge, Morgan et al.: "Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment." in: Nature methods, Vol. 2, Issue 1, pp. 47-53, 2005 (PubMed).

Taylor, Noguchi, Totoki et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification...." in: Nature, Vol. 440, Issue 7083, pp. 497-500, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Hockert, Yeh, MacDonald: "The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 695-704, 2009 (PubMed).

Hosts (21), (3), (2)
Reactivities (24), (15), (15), (14), (14), (14), (2), (2), (1)
Applications (17), (10), (7), (5), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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