Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) antibody

Antigen: Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3)

Synonyms: cstf3, MGC76035, MGC83109, CSTF3, DKFZp468C1613, CSTF-77, MGC43001, MGC75122, MGC117398, C79532, CstF-77, MGC6373, 4732468G05Rik, MGC156746, zgc:56028, wu:fa99c02, wu:fb18b09

Clonality: Monoclonal (1D4)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395003

Additional Information

Alternative name: CSTF3

Gene ID: 1479

Immunogen: CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1D4

Description: Other names: MGC75122 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 82922 DA

Comments:

Background: The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to CSTF3 (AT1663a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi ( Cat # H00001479-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody (M01), clone 1D4 (Cat # AT1663a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) antibody (Image 2)

Publications

Product: Kim, Bhinge, Morgan et al.: "Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment." in: Nature methods, Vol. 2, Issue 1, pp. 47-53, 2005 (PubMed).

Taylor, Noguchi, Totoki et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification." in: Nature, Vol. 440, Issue 7083, pp. 497-500, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Hockert, Yeh, MacDonald: "The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 695-704, 2009 (PubMed).