alpha-Methylacyl-CoA Racemase (AMACR) antibody

Antigen: alpha-Methylacyl-CoA Racemase (AMACR)

Synonyms: RACE, CBAS4, Macr1, Da1-8, Marc1, AMACR, DKFZp469O1232, amacr, MGC89832

Clonality: Monoclonal (1D8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395030

Additional Information

Alternative name: AMACR

Gene ID: 23600

Immunogen: AMACR (AAH09471, 1 a.a. ~ 199 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 1D8

Description: Other names: RACE alpha-methylacyl-CoA racemase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AMACR (AAH09471, 1 a.a. ~ 199 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 42360 DA

Comments:

Background: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Structure

Restrictions: For Research Use only

Images

AMACR monoclonal antibody (M02), clone 1D8. Western Blot analysis of AMACR expression in HepG2 ( Cat # L019V1 ).

Publications

Product: Hosgood, Menashe, He et al.: "PTEN identified as important risk factor of chronic obstructive pulmonary disease." in: Respiratory medicine, Vol. 103, Issue 12, pp. 1866-70, 2009 (PubMed).

Halsey, Calder, Mathew et al.: "Expression of alpha-methylacyl-CoA racemase (P504S) in sebaceous neoplasms." in: Journal of cutaneous pathology, Vol. 37, Issue 4, pp. 446-51, 2010 (PubMed).

Guey, García-Closas, Murta-Nascimento et al.: "Genetic susceptibility to distinct bladder cancer subphenotypes." in: European urology, Vol. 57, Issue 2, pp. 283-92, 2010 (PubMed).

Szponar, Beothe, Kovacs: "How useful is alpha-methylacyl-CoA racemase (AMACR) immunohistochemistry in the differential diagnosis of kidney cancers?" in: Histopathology, Vol. 56, Issue 2, pp. 263-5, 2010 (PubMed).

Lakis, Papamitsou, Panagiotopoulou et al.: "AMACR is associated with advanced pathologic risk factors in sporadic colorectal adenomas." in: World journal of gastroenterology : WJG, Vol. 16, Issue 20, pp. 2476-83, 2010 (PubMed).