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Apolipoprotein M (APOM) (AA 23-189) antibody

Details for Product No. ABIN395065
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Antigen
Synonyms APOM, fb62d12, wu:fb62d12, g3a, ng20, hspc336, G3a, HSPC336, NG20, apo-M, 1190010O19Rik
Epitope
AA 23-189
(10), (9), (7), (5), (4), (3), (2), (2), (1), (1)
Reactivity
Human
(66), (32), (17), (15), (13), (12), (1)
Host
Mouse
(45), (33), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(73), (55), (19), (16), (15), (13), (10), (7), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN395065
450.00 $
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Immunogen APOM (AAH20683, 23 a.a. ~ 189 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1F10
Isotype IgG1 kappa
Specificity APOM (AAH20683, 23 a.a. ~ 189 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name APOM
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 21253 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Apolipoprotein M (APOM) (AA 23-189) antibody Western blot analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10. Lane 1: APOM transfected lysate(21.3 KDa). Lane 2: Non-transfected lysate.
anti-Apolipoprotein M (APOM) (AA 23-189) antibody (2) anti-Apolipoprotein M (APOM) (AA 23-189) antibody (Image 2)
Background publications Ahnström, Gottsäter, Lindblad et al.: "Plasma concentrations of apolipoproteins A-I, B and M in patients with abdominal aortic aneurysms." in: Clinical biochemistry, Vol. 43, Issue 4-5, pp. 407-10, 2010 (PubMed).

Han, Jia, Jia: "WITHDRAWN: Association of apolipoprotein M gene polymorphisms with cerebral infarction and lipid levels in a Chinese Han population." in: Neuroscience, 2010 (PubMed).

Ahnström, Gottsäter, Lindblad et al.: "Plasma concentrations of apolipoproteins A-I, B, and M in patients with critical limb ischemia." in: Clinical biochemistry, Vol. 43, Issue 6, pp. 599-603, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Validation Images
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