Apolipoprotein M (APOM) antibody

Antigen: Apolipoprotein M (APOM)

Synonyms: G3a, NG20, HSPC336, MGC22400, 1190010O19Rik, APOM, MGC128780, fb62d12, wu:fb62d12, g3a, ng20, hspc336, DKFZp469N144

Clonality: Monoclonal (1F10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395065

Additional Information

Alternative name: APOM

Gene ID: 55937

Immunogen: APOM (AAH20683, 23 a.a. ~ 189 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1F10

Description: Other names: G3a, HSPC336, MGC22400, NG20 apolipoprotein M

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: APOM (AAH20683, 23 a.a. ~ 189 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 21253 DA

Comments:

Background: The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi ( Cat # H00055937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with APOM monoclonal antibody (M01), clone 1F10 (Cat # AT1171a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10. Lane 1: APOM transfected lysate(21.3 KDa). Lane 2: Non-transfected lysate.

anti-Apolipoprotein M (APOM) antibody (Image 2)

Publications

Product: Ahnström, Gottsäter, Lindblad et al.: "Plasma concentrations of apolipoproteins A-I, B and M in patients with abdominal aortic aneurysms." in: Clinical biochemistry, Vol. 43, Issue 4-5, pp. 407-10, 2010 (PubMed).

Han, Jia, Jia: "WITHDRAWN: Association of apolipoprotein M gene polymorphisms with cerebral infarction and lipid levels in a Chinese Han population." in: Neuroscience, 2010 (PubMed).

Ahnström, Gottsäter, Lindblad et al.: "Plasma concentrations of apolipoproteins A-I, B, and M in patients with critical limb ischemia." in: Clinical biochemistry, Vol. 43, Issue 6, pp. 599-603, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).