|+1 404 474 4654|
|+1 888 205 9894 (TF)|
RAS P21 Protein Activator 3 (RASA3) antibody
|Synonyms||GAPIII, MGC46517, MGC47588, GAP1IP4BP, C86362, AI326412, E130011G04, R-ras gap, RASA3, rasa3, MGC82492, gapiii, gap1ip4bp, LOC100222817|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||RASA3 (AAH47242, 725 a.a. ~ 834 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: GAP1IP4BP, GAPIII, MGC46517, MGC47588 RAS p21 protein activator 3|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||RASA3 (AAH47242, 725 a.a. ~ 834 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||95699 DA|
Background: The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure, Cell Cycle|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged RASA3 is approximately 0.3ng/ml as a capture antibody.|
Walker, Kupzig, Lockyer et al.: "Analyzing the role of the putative inositol 1,3,4,5-tetrakisphosphate receptor GAP1IP4BP in intracellular Ca2+ homeostasis." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48779-85, 2002 (PubMed).
Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).
Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).
Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).
Kupzig, Bouyoucef-Cherchalli, Yarwood et al.: "The ability of GAP1IP4BP to function as a Rap1 GTPase-activating protein (GAP) requires its Ras GAP-related domain and an arginine finger rather than an asparagine thumb." in: Molecular and cellular biology, Vol. 29, Issue 14, pp. 3929-40, 2009 (PubMed).
|Hosts||Rabbit (23), Goat (3), Mouse (1)|
|Reactivities||Human (23), Mouse (Murine) (21), Rat (Rattus) (21), Pig (Porcine) (19), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18)|
|Applications||Immunofluorescence (IF) (15), ELISA (10), Western Blotting (WB) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Internal Region (1)|