RAS P21 Protein Activator 3 (RASA3) antibody

Antigen: RAS P21 Protein Activator 3 (RASA3)

Synonyms: GAPIII, MGC46517, MGC47588, GAP1IP4BP, C86362, AI326412, E130011G04, R-ras gap, RASA3, rasa3, MGC82492, gapiii, gap1ip4bp, LOC100222817

Clonality: Monoclonal (1F11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395074

Additional Information

Alternative name: RASA3

Gene ID: 22821

Immunogen: RASA3 (AAH47242, 725 a.a. ~ 834 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1F11

Description: Other names: GAP1IP4BP, GAPIII, MGC46517, MGC47588 RAS p21 protein activator 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RASA3 (AAH47242, 725 a.a. ~ 834 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 95699 DA

Comments:

Background: The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged RASA3 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Walker, Kupzig, Lockyer et al.: "Analyzing the role of the putative inositol 1,3,4,5-tetrakisphosphate receptor GAP1IP4BP in intracellular Ca2+ homeostasis." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48779-85, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Kupzig, Bouyoucef-Cherchalli, Yarwood et al.: "The ability of GAP1IP4BP to function as a Rap1 GTPase-activating protein (GAP) requires its Ras GAP-related domain and an arginine finger rather than an asparagine thumb." in: Molecular and cellular biology, Vol. 29, Issue 14, pp. 3929-40, 2009 (PubMed).