S100 Calcium Binding Protein A4 (S100A4) antibody

Antigen: S100 Calcium Binding Protein A4 (S100A4)

Synonyms: 42A, 18A2, CAPL, FSP1, MTS1, P9KA, PEL98, 42a, Capl, FSp1, Mts1, pk9a, PeL98, metastasin, S100A4, P9ka, RNP9KA

Clonality: Monoclonal (1F12-1G7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395081

Additional Information

Alternative name: S100A4

Gene ID: 6275

Immunogen: S100A4 (AAH16300, 1 a.a. ~ 102 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1F12-1G7

Description: Other names: 18A2, 42A, CAPL, MTS1, P9KA, PEL98S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: S100A4 (AAH16300, 1 a.a. ~ 102 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 11729 DA

Comments:

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to S100A4 ( Cat# AT3757a ) on HeLa cell . [antibody concentration 15 ug/ml] Western Blot analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody (M01), clone 1F12-1G7. Lane 1: S100A4 transfected lysate(11.7 KDa). Lane 2: Non-transfected lysate.

anti-S100 Calcium Binding Protein A4 (S100A4) antibody (Image 2)

Publications

Product: Roberts, Kawut, Krowka et al.: "Genetic risk factors for hepatopulmonary syndrome in patients with advanced liver disease." in: Gastroenterology, Vol. 139, Issue 1, pp. 130-9.e24, 2010 (PubMed).

Zhang, Fu, Chen et al.: "Subcellular distribution of S100A4 and its transcriptional regulation under hypoxic conditions in gastric cancer cell line BGC823." in: Cancer science, Vol. 101, Issue 5, pp. 1141-6, 2010 (PubMed).

Cunningham, Docherty, Burke et al.: "S100A4 expression is increased in stricture fibroblasts from patients with fibrostenosing Crohn's disease and promotes intestinal fibroblast migration." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 2, pp. G457-66, 2010 (PubMed).

Mencía, Selga, Rico et al.: "Overexpression of S100A4 in human cancer cell lines resistant to methotrexate." in: BMC cancer, Vol. 10, pp. 250, 2010 (PubMed).

van Dieck, Lum, Teufel et al.: "S100 proteins interact with the N-terminal domain of MDM2." in: FEBS letters, Vol. 584, Issue 15, pp. 3269-74, 2010 (PubMed).